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Mitochondria in Pleurotus ostreatus were isolated and purified by the stepped sucrose density gradient centrifugation, to investigate the effects of the light on the enzymatic activity of the mitochondrial ATP synthase. This enzyme, which was illuminated by the light ranging from 400 ㎚ to 700 ㎚, showed that the specific activity was stimulated at 490 ㎚ for 15 sec. Effects of organic compounds on the mitochondrial ATP synthase were also investigated at the optimum conditions; The activities of this enzyme were increased to 168 percent by the addition of 2,6-dichlorophenol indophenol(DCPIP), 224 percent by phenazine methosulfate(PMS), but inhibited 91 percent by oligomycin, 14 percent by 2-heptyl-4-hydroxyquinoline-N-oxide(HQNO) and 75 percent by 2,4-dinitrophenol (DNP), respectively. Effects of metal ions of the mitochondrial ATP synthase were investigated at the optimum conditions. The activities of the enzyme were inhibited 35 percent by Ca^(2+), 14 percent by Co^(2+) and 73 percent by Mn^(2+). For effects of anions, the activities of this enzyme were inhibited 80 percent by CN^-, 52 percent by SO₄^(2+), 28 percent by each of CO₃^(2+) and NO₃^-, respectively.
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